Morphological, physiological and antioxidant response of Stevia rebaudiana under in vitro agar induced drought stress
2023
Soheila Lahijanian | Morteza Eskandari | Ghasem Akhbarfar | Iraj Azizi | Marziyeh Afazel | Cyrus Ghobadi
In this investigation, the response of Stevia rebaudiana Bertoni was studied in different in vitro drought conditions. A method based on agar concentration in different levels (6, 8, 10, 12, 14 gl-1) was used to induce some drought stress levels. Agar concentrations can make a meaningful difference in biochemical, physiological, and antioxidant responses of the Stevia. Under the stress condition, the highest accumulation of proline content (50%) and malondialdehyde (MDA) (42%) were recorded in the highest concentration of agar. Moreover, antioxidant enzyme activities, ascorbate peroxidase (17%), catalase (53%), and peroxidase (30%) have been drastically enhanced from 8 gl-1 agar. Current results indicated that using agar as a water stress agent could be effectively exploited as an efficient method in modeling the in vitro drought stress in Stevia rebaudiana.
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