液相色谱法检测鱼类肝脏中天然维生素ADetermination of natural vitamin A in fish liver by liquid chromatography
2022
黄文婷1，曹文红1, 2, 3, 4, 5, 6, 7，章超桦1, 2, 3, 4, 5, 6, 7，秦小明1, 2, 3, 4, 5, 6, 7，高加龙1, 2, 3, 4, 5, 6, 7，郑惠娜1, 2, 3, 4, 5, 6, 7 HUANG Wenting1, CAO Wenhong1,2,3,4,5,6,7, ZHANG Chaohua1,2,3,4,5,6,7, QIN Xiaoming1,2,3,4,5,6,7, GAO Jialong1,2,3,4,5,6,7, ZHENG Huina1,2,3,4,5,6,7
为了准确测定鱼类肝脏中维生素A（维生素A1、维生素A2）含量，对样品预处理方法（水浴皂化法、室温皂化法、直接提取法）和检测方法（正相色谱法、反相色谱法）进行筛选，并应用于9种经济鱼类肝脏中维生素A的测定。结果表明：维生素A1、维生素A2标准品用反相色谱法检测分离效果良好，且在各自线性范围内线性关系良好，R2均大于0.99；水浴皂化法维生素A含量显著高于室温皂化法及直接提取法（p<0.05），且维生素A1平均回收率为104.52%，维生素A2平均回收率为90.94%；在9种鱼中，除了淡水鱼乌鳢和大口黑鲈外，其他淡水、海水鱼类肝脏中维生素A总含量均达200 μg/100 g以上，其中海水鱼龙趸石斑鱼肝脏中维生素A总含量最高，达到14 413.78 μg/100 g。水浴皂化法结合反相色谱法精密度良好，适用于鱼类肝脏中维生素A的分析测定。 In order to accurately determine the content of vitamin A (vitamin A1, vitamin A2) in fish liver, sample pretreatment methods (water bath saponification, room temperature saponification, direct extraction) and detection methods (normal phase chromatography, reversed phase chromatography) were screened and applied to the determination of vitamin A in the liver of nine economic fish species. The results showed that vitamin A1 and vitamin A2 were separated effectively by reversed phase chromatography and showed good linear relationship within their respective linear ranges (R2 > 0.99); the content of vitamin A extracted by water bath saponification was significantly higher than that by room temperature saponification and direct extraction (p<0.05), and its average recoveries of vitamin A1 and vitamin A2 were 104.52% and 90.94%, respectively. Except for the freshwater snakehead and big mouth bass, the total content of vitamin A in the livers of other freshwater fishes and marine fishes was more than 200 μg/100 g, and the total content of vitamin A in the liver of marine giant grouper was the highest, reaching 14 413.78 μg/100 g. The water bath saponification method combined with reversed phase chromatography has good precision and is suitable for the determination of vitamin A in fish liver.
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