Produksi dan uji aktivitas enzim fatty acid photodecarboxylase dari Chlorella variabilis melalui kultivasi pada medium CYT dan KW21
2022
Yora Faramitha | Sheren P Paramita | Fauziatul Fitriyah | Djoko Santoso | Irma Kresnawaty
Chlorella variabilis is a microalgae that produce fatty acid photodecarboxylase (CvFAP) which can catalyze the decarboxylation of long chain fatty acids into hydrocarbons through a process influenced by blue light. However, CvFAP enzyme content in C. variabilis is relatively small and the ability of the enzyme as a biocatalyst needs to be optimized. This study aimed to compare the effect of C. variabilis microalgae cultivation medium types: C medium, yeast extract, and tryptone (CYT medium) and KW21 medium which is a commercial marine algae culture medium, on microalgae growth and the resulting protein. In addition, the CvFAP enzyme extract from each medium was tested for its ability to convert long-chain fatty acids into pentadecane by optimizing the substrate type, enzyme concentration, and incubation time. C. variabilis was cultivated for 2-3 weeks with a ratio of light and dark period of 8:16 (hours/hours). The protein content of C. variabilis was determined using the Lowry method. Proteins were extracted physico-chemically and enzyme activity assay were carried out under exposure to blue light. The pentadecane content formed from the enzyme activity assay was measured using GC-MS. The study results showed that microalgae growth and protein content of C. variabilis were higher in the CYT medium compare to those in KW21 medium. The protein band appears at 67-68 kDA which is speculated to affect the ternary complex bond between FAP-FAD-FA, which is a determinant of the results in the enzyme activity assay process in accumulating hydrocarbons. Meanwhile, the results of CvFAP enzyme activity assay showed that the CvFAP enzyme extracted from KW21 medium produced higher concentration of pentadecane (2.8 times) than from CYT medium.
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