Analysis of Interplanting Soybean under Kiwifruit Forest on Soil Microbial Community Structure

【Objective】This study aims to reveal the effects of interplanting soybean under kiwifruit forests on soil microbial community structure, and analyze the changes in soil microbial community structure under kiwifruit plantations with and without soybeans, so as to provide theoretical basis and technical support, for studying the soil microecological improvement and green planting of kiwifruit, as well as the improvement of economic benefits per unit area of land in remote mountainous areas.【Method】The abundance of microbial communities in different soil samples under kiwifruit forests was detected by using high-throughput sequencing. The differences in microbial abundance and structural composition in soil samples under kiwifruit forests and those interplanted with soybeans were analyzed. The impact of interplanting soybeans under kiwifruit forests on the structure and diversity of soil microbial communities were explored. Firstly, the soils planting with soybeans under kiwifruit forest and the soils non-planting with soybeans were collected for pretreatment, DNA extraction and PCR amplification, and then the products were detected, purified and quantified based on Illumina MiSeq high-throughput sequencing method, and database for sequencing was built. The community classification structure, microbial abundance and diversity, and the dominant flora species were determined by data analysis【Result】The quality control and statistical results of Tags abundance and OTU showed that the microbial abundance and diversity in soils planting soybean (EK) were higher than non-planting soybean (CK). The diversity analysis showed the composition of microbial communities exhibited significant differences between EK and CK. The analysis of microbial community structure showed that the average proportions of EK and CK at family, genus, and species levels are 27.06% and 28.11%, 32.66% and 29.33%, 0.46% and 0.37%, respectively, and the soil microbial community of EK was higher than that of CK at genus and species levels. A total of 651 883 valid sequences were obtained from 6 soil samples, and the 4 740 and 4 551 OTUs were obtained by clustering for EK and CK, respectively. The LEfSe analysis showed that there were 19 dominant microbial groups in CK, while 41 dominant microbial groups in EK. Among them, Proteobacteria was the dominant phylum and Ensifer adhaerens, a member of the Rhizobiaceae family, was the dominant bacterium of EK.【Conclusion】The structure of soil microbial community was changed when soybean were interplaned under kiwifruit forests, and the abundance and diversity of soil microbial community, as well as the number of dominant and beneficial microbial communities in soils were increased. In summary, intercropping soybeans under kiwifruit forests has an important effect on improving the soil effective components and increasing soil fertility.