Functional Analysis of <i>ScABI3</i> from <i>Syntrichia caninervis</i> Mitt. in <i>Medicago sativa</i> L.
2022
Yi Zhang | Chun Wang | Mingqi Huang | Yigong Zhang
ABI3 (ABSCISIC ACID INSENSITIVE 3) is a family of B3 transcription factors essential for regulating the abscisic acid (ABA) signaling pathway involved in various biological processes and abiotic stress. Our previous studies demonstrated that ectopic expression of <i>ScABI3</i> from a desiccation-tolerant moss (<i>Syntrichia caninervis</i>) into <i>Arabidopsis thaliana</i> enhanced abiotic stress tolerance. However, studies on plant transformation using the <i>ABI3</i> gene are limited and other possible functions of <i>ScABI3</i> are not known. Here, we transformed the <i>ScABI3</i> into alfalfa (<i>Medicago sativa</i> L.) and analyzed the effects on phenotype, photosynthetic efficiency, and nutritional quality. The results showed that the endogenous ABA content of the transgenic plants was significantly higher than WT, and the leaf-stem ratio, leaf area, and branch number increased with <i>ScABI3</i> overexpression in alfalfa. Further analysis of the gas exchange parameters showed that the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and water-use efficiency (WUE) of the transgenic alfalfa were significantly higher than WT; meanwhile, the protein content of the transgenic lines was higher than the WT, but the crude fat content was lower. Thus, these findings suggest that <i>ScABI3</i> can be used as a potential candidate gene to manipulate alfalfa’s growth and nutritional quality. This study will provide a theoretical basis for breeding alfalfa varieties and assist in forage production and animal husbandry in the future.
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