TECHNOLOGY OF PRODUCING SYMBIOTIC BIOLOGICALLY ACTIVE ADDITIVE

<p>The article presents data on the development of the technology of multicomponent probiotics from two bacterial strains: <em>Bifidobacterium longum-Ya3</em> and <em>Propionibacterium shermanii-4</em>. The ability of bacteria of the genus <em>Propionibacterium</em> to have a selective stimulating effect on the growth of bacteria of the genus <em>Bifidobacterium </em>has been characterised. Based on the experimental data obtained with the MATLAB software, optimal conditions were determined for the accumulation of the maximum amount of biomass of the consortium of <em>Bifidobacterium</em><em> longum-Ya3</em> and <em>Propionibacterium shermanii-4</em>. The main parameters that determined the yield of biomass in the process of cultivation under different temperature conditions (T=30°С, Т=34 °С, Т=37 °С) have been taken as the optimality criteria. These parameters are the number of colony-forming units and the active acidity. It has been established that the optimal time for cultivating a consortium of bifidobacteria and propionibacteria in a soy-lactose medium is 24 hours at a temperature of 34°C. On the basis of the data obtained, we have created a symbiotic BAA (biologically active additive) and developed a basic technological scheme for its production. The biologically active additive was created on the basis of the symbiotic consortium of bifido and propionibacteria containing 4 × 10¹⁰ CFU/cm³ of <em>B. longum-Ya3</em>, and 3 × 10¹⁰ CFU/cm³ of <em>P. shermanii-4</em>. The microbiological control of the quality of the obtained dietary supplement based on the consortium of the bacteria <em>Bifidobacterium longum-Ya3</em> and <em>Propionibacterium shermanii-4</em> has found no pathogenic and sanitary indicator microorganisms. It means that the finished product is safe and suitable for consumption. As for the organoleptic parameters, the BAA obtained is of a powder-like structure, beige-coloured, with a specific taste and smell.</p>