Assessment of genetic diversity of fennel ecotypes using RAPD and ISSR markers
2016
Safoura Taheri | Mohammd Zabet | Ali Izanlou | Ali Izadi Darbandi
Fennels have long been cultivated as an aromatic and medicinal plant in different regions with different climate in Iran. In this study, the genetic diversity of 32 ecotypes of fennel was assessed by RAPD and ISSR markers. A total of 106 and 72 polymorphic fragments were amplified by RAPD and ISSR markers, respectively. Based on RAPD data, mean genetic diversity were 0.3739 and 0.552 by Nei (h) and Shannon's information index (I), respectively. Moreover, mean genetic diversity for ISSR marker were accordingly 0.2944 and 0.4563. The obtained average polymorphism content (PIC) by RAPD and ISSR markers were 0.4274 and 0.2192, respectively. Based on cluster analysis of RAPD and ISSR markers, the ecotypes were grouped in 7 and 11 clusters. These results indicate that the Iranian fennels have high genetic diversity, and RAPD and ISSR markers very well differentiated fennel genotypes based on the geographical distribution and climatic similarities. Understanding the genetic diversity can further help breeders in their breeding programs, especially in hybridization breeding, evolutionary and classifications studies.
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