Genetic Analysis and Gene Mapping for a Short-Petiole Mutant in Soybean (<i>Glycine max </i>(L.)<i> </i>Merr<i>.</i>)
2019
Meifeng Liu | Yaqi Wang | Junyi Gai | Javaid Akhter Bhat | Yawei Li | Jiejie Kong | Tuanjie Zhao
Short petiole is a valuable trait for the improvement of plant canopy of ideotypes with high yield. Here, we identified a soybean mutant line <i>derived short petiole</i> (<i>dsp</i>) with extremely short petiole in the field, which is obviously different from most short-petiole lines identified previously. Genetic analysis on 941 F<sub>2</sub> individuals and subsequent segregation analysis of 184 F<sub>2:3</sub> and 172 F<sub>3:4 </sub>families revealed that the <i>dsp </i>mutant<i> </i>was controlled by two recessive genes, named as <i>dsp1</i> and <i>dsp2</i>. Map-based cloning showed that these two recessive genes were located on two nonhomologous regions of chromosome 07 and chromosome 11, of which the<i> dsp1 </i>locus was mapped at a physical interval of 550.5-Kb on chromosome 07 near to centromere with flanking markers as BARCSOYSSR_07_0787 and BARCSOYSSR_07_0808; whereas, the <i>dsp2</i> locus was mapped to a 263.3-Kb region on chromosome 11 with BARCSOYSSR_11_0037 and BARCSOYSSR_11_0043 as flanking markers. A total of 36 and 33 gene models were located within the physical genomic interval of <i>dsp1 </i>and <i>dsp2 </i>loci, respectively. In conclusion, the present study identified markers linked with genomic regions responsible for short-petiole phenotype of soybean, which can be effectively used to develop ideal soybean cultivars through marker-assisted breeding.
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