DESINFESTAÇÃO IN VITRO DA BANANEIRA ‘FARTA VELHACO (SUB GRUPO AAB)’ EM DIFERENTES CONCENTRAÇÕES DE CLORO ATIVO
2015
GUSTAVO ALVES PEREIRA | MARCÍLIO BOBROFF SANTAELLA | LEFAYETE MICHELE SANTANA MONTENEGRO ALVES | ELDER CASSIMIRO DA SILVA
Most banana plantations are still made using traditional clones per type horn and rhizomes. Oth- er propagation methods such as micropropagation have been developed and perfected to raise the rate of multi- plication in a short time and improve the health status of the seedlings. However, contamination by bacteria and fungi is one of the biggest problems of this technique. The aim was to evaluate the in vitro disinfestation using active chlorine concentrations of banana explants ‘Farta Velhaco’. The experimental design used was com- pletely randomized consisting of five treatments and five repetition, each replicate represented by five explants in different concentrations of active chlorine for twenty minutes: T1 - without active chlorine, T2 - 0.5% active chlorine, T3 - 1.0% of active chlorine, T4 - 1.5% active chlorine and T5 - 2% active chlorine. The percentages of contamination by bacteria and fungi as well as the percentage of oxidation of the explants were evaluated. Data were subjected to analysis of variance and regression analysis. The results showed that the highest effi- ciency among the tested treatments was the immersion of explants in 1% of active chlorine that caused a reduc- tion in 95% and 90% respectively for bacteria and fungi and 60% of explants oxidation. It was concluded that this concentration can be used to control contamination for micropropagation of banana 'Farta Velhaco'.
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