Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication
2010
Nikita O. Ronzhin | Konstantin A. Kharin | Alexey P. Puzyr | Vladimir S. Bondar
This paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial cells: the process requires minimum equipment and time; one technological cycle yields 40-45 % of desired product with high-quality (according to the SDS-PAGE). The application of nanodiamonds for design of indicating systems is demonstrated on the example of a biosensor system based on the nanodiamondluciferase complex and inert polymeric matrix.
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