Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
2018
Ashraf K. Sulieman | Meilana Dharma Putra | Ahmed E. Abasaeed | Mohamed H. Gaily | Saeed M. Al-Zahrani | Mohamed A. Zeinelabdeen
Background: Ethanol and fructose are two important industrial products that enjoy many uses. In this contribution, their production via selective fermentation of date extract using Saccharomyces cerevisiae was studied. Scaling up the process for possible commercialization was investigated in three fermentors with working volume ratio of 1:40:400. Results: Higher ethanol concentration was obtained in the larger fermentor due to conversion of fructose. Fructose yields in the 0.5-L, 7.5-L and 80-L fermentors were 99, 92 and 90%, respectively. Good fitting was obtained with the modified Monod kinetics; however, a better fit of cell mass was obtained with the modified Ghose–Tyagi model which accounts for ethanol inhibition. Conclusions: The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.How to cite: Sulieman AK, Putra MD, Abasaeed AE, et al. Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.04.006. Keywords: Bioreactor, Biosynthesis of ethanol, Ethanol, Fermenters, Fructose, Kinetic modeling, Kinetic models for ethanol biosynthesis, Kinetic models for fructose biosynthesis, Microbiological biosynthesis, Saccharomyces cerevisiae, Yeast
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