Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide
2023
Susmit Mhatre | Anjali Rai | Hatim Ali | Akash Patil | Neetu Singh | Richa Verma | John Auden | Cole Chandler | Alekha Dash | Catherine Opere | Somnath Singh
Background: Hydrogen sulfide (H2S), an endogenous gasotransmitter, has potential applications in several conditions. However, its quantification in simulated physiological solutions is a major challenge due to its gaseous nature and other physicochemical properties. Aim: This study was designed to compare four commonly used H2S detection and quantification methods in aqueous solutions. Methods: The four techniques compared were one colorimetric, one chromatographic and two electrochemical methods. Results: Colorimetric and chromatographic methods quantified H2S in millimolar and micromole ranges, respectively. The electrochemical methods quantified H2S in the nanomole and picomole ranges and were less time-consuming. Conclusion: The H2S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.
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