Potencial probiótico de cepas nativas para uso como aditivos en la alimentación avícola
2012
Cecilia Lara Mantilla | Ángela Burgos Portacio
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mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} </style> <![endif]--> <p class="MsoNormal" style="text-align: justify;"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Título en ingles: Probiotic potential of native strains, for as feed additives for poultry </span></strong></span><strong style="mso-bidi-font-weight: normal;"></strong></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt;">Resumen: </span></strong><span style="font-size: 10.0pt;">Se evaluó <em style="mso-bidi-font-style: normal;">in vitro</em> el <span style="color: #231f20;">potencial probiótico de cepas nativas aisladas de las heces de pollos asilvestrados (<em style="mso-bidi-font-style: normal;">Gallus gallus)</em> pertenecientes a los géneros <em>Lactobacillus </em>sp,<em style="mso-bidi-font-style: normal;"> Bacillus</em> sp y levaduras tipo <em style="mso-bidi-font-style: normal;">saccharomyces sp</em>; se determinó la actividad probiótica mediante pruebas</span> de resistencia al ácido (pH<span style="mso-spacerun: yes;"> </span>3, 4, 5, 6, 7), sales de bilis (0,05, 0,1, 0,15, 0.3 %), tolerancia al NaCl (2, 4, 7, 10 %), actividad antagónica (<em style="mso-bidi-font-style: normal;">Salmonella sp</em>, <em style="mso-bidi-font-style: normal;">E. coli</em>), determinación del tipo de fermentación, crecimiento a temperaturas (28,37,43°C) y capacidad de crecimiento. Las cepas con mayor tolerancia se identificaron a través de pruebas bioquímicas y fermentación de carbohidratos. Como resultado se observó que tres microorganismos:<em style="mso-bidi-font-style: normal;"><span style="color: #231f20;"> Saccharomyces </span></em><span style="color: #231f20;">sp. (3),<em> </em><em style="mso-bidi-font-style: normal;">Bacillus</em> sp. </span></span><span style="font-size: 10.0pt; color: #231f20; mso-ansi-language: EN-US;" lang="EN-US">(7) y <em>Lactobacillus </em>sp. (14)<em style="mso-bidi-font-style: normal;"> </em></span><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">poseen propiedades<em> </em><span style="mso-bidi-font-style: italic;">probióticas. </span></span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Palabras claves: </span></strong><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Gallus gallus; <span style="color: #231f20;">Saccharomyces sp.</span></span></em><span style="font-size: 10.0pt; color: #231f20; mso-ansi-language: EN-US;" lang="EN-US">; <em> </em><em style="mso-bidi-font-style: normal;">Bacillus</em> <em style="mso-bidi-font-style: normal;">sp.; </em><em>Lactobacillus </em><em style="mso-bidi-font-style: normal;">sp.; </em></span><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">aditivos <span style="mso-spacerun: yes;"> </span>microbianos.</span></p> <p class="MsoNormal" style="text-align: justify;"><span class="longtext"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; background: white; mso-ansi-language: EN-US;" lang="EN-US">Abstract: </span></strong></span><span class="hps"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Was evaluated <em style="mso-bidi-font-style: normal;">in vitro</em> the probiotic potential of native strains isolated from feces of wild chickens (<em style="mso-bidi-font-style: normal;">Gallus gallus</em>) belonging to the genera <em style="mso-bidi-font-style: normal;">Lactobacillus sp., Bacillus sp. and Saccharomyces</em> ; probiotic activity was determined by testing acid resistance (pH 3, 4, 5.6, 7), bile salts (0,05, 0,1, 0,15, 0,3%), tolerance to NaCl (2, 4, 7,10%), antagonistic activity (<em style="mso-bidi-font-style: normal;">Salmonella spp, E. coli</em>), production gas (glucose), growth temperatures (28, 37, 43 ° C) and growth capacity. The most tolerant strains were identified by biochemical tests and carbohydrate fermentation. As a result it was found that three microorganisms: <em style="mso-bidi-font-style: normal;">Saccharomyces sp. (3), Bacillus sp. (7) and Lactobacillus sp.</em> (14) have probiotic properties. </span></span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Key words:</span></strong><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US"> Gallus gallus; <span style="color: #231f20;">Saccharomyces sp.; </span></span></em><span style="font-size: 10.0pt; color: #231f20; mso-ansi-language: EN-US;" lang="EN-US"><em> </em><em style="mso-bidi-font-style: normal;">Bacillus</em> <em style="mso-bidi-font-style: normal;">sp.; </em><em>Lactobacillus </em><em style="mso-bidi-font-style: normal;">sp.; </em></span><span class="hps"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">microbial additives</span></span><span class="longtext"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">.</span></span><strong style="mso-bidi-font-weight: normal;"></strong></p>
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