Characterization of pinctada fucata mantle gene 2 (pfmg2) in pearl oyster
2017
Xiaoyan Wang | Liyuan Li | Shangfeng Liu | Zhao Wang
Biomineralization is a complicated process which involves multiple gene products in Pinctada fucata. Here, Pinctada fucata mantle gene 2 (pfmg2) was characterized in P. fucata. The putative PFMG2 protein has a Calponin Homology (CH) domain and an EF hand that can bind calcium. PFMG2 shares homology with transgelin in other species. Multi-tissue reverse transcriptase polymerase chain reaction (RT-PCR) indicates that pfmg2 mRNA is expressed ubiquitously, but its expression is high in the mantle tissue of P. fucata. Furthermore, in situ hybridization shows that pfmg2 localized at the edge of the mantle. Our results suggest that PFMG2 plays an important role in the shell mineralization process in pearl oyster. The analysis and the functional characterization of PFMG2 in P. fucata will provide new insight into the mineralization process.
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