THE PHENOLS ACCUMULATION IN TRANSFORMED ROOT CULTURES OF DIFFERENT EXPLANTS SOURCES OF COMMON BUCKWHEAT (Fagopyrum esculentum Moench)
2013
O. V. Sytar | A. M. Gabr | N. Yu. Taran | I. M. Smetanska
The growth parameters of transformed root cultures, total phenolic content and phenolic acids composition has been studied in root cultures, which were obtained from various explants of buckwheat by Agrobacterium rhizogenes strains A4. The methods of obtaining of the transformed root cultures, total phenol estimation, gas-liquid chromatography and polymerase chain reaction has been used. Elevated levels of total phenols in transformed roots of buckwheat from different sources of explants have been found. The high content of chlorogenic, p-hydroxybenzoic, p-anisic and caffeic acids has been discovered in the root cultures, which can be used for their industrial production. Maximal root growth was equal 21.2 g/l of dry weight in the roots as source for root culture, 17.7 g/l with leaves and 14.6 g/l with stems at 3 week after placement. Molecular analysis by polymerase chain reaction amplification was confirmed that the rol B gene (652 bp) which transferred info hairy roots from Ri-plasmid in Agrobacterium rhizogenes is responsible for induction of root from plant species.
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