Optimizing Antioxidant Activity and Phytochemical Properties of Peppermint (<i>Mentha piperita</i> L.) by Integrative Application of Biofertilizer and Stress-Modulating Nanoparticles under Drought Stress Conditions

Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO₂ NPs on the nutrient uptake, dry yield, essential oil (EO) productivity, and EO quality of peppermint (<i>Mentha piperita</i> L.) under different irrigation regimes. The treatments included three irrigation regimes containing irrigation after 20% (I₂₀, well-watered), 40% (I₄₀, mild DS), and 60% (I₆₀, severe DS) maximum allowable depletion (MAD) percentage of the soil’s available water as well as four fertilizer sources contain no fertilization (control), Myco-Root biofertilizer, TiO₂ NPs, and an integrative application of Myco-Root + TiO₂ NPs. The results demonstrated that the highest (195.72 g m⁻²) and the lowest dry yield (78.76 g m⁻²) of peppermint was obtained in well-watered conditions with integrative application of Myco-Root + TiO₂ NPs and severe drought stress (I₆₀) without fertilization, respectively. The dry yield of peppermint was reduced by 27.7 and 53.4% in mild (I₄₀) and severe drought stress (I₆₀), respectively. The maximum EO content (1.49%) and EO yield (2.30 g m⁻²) was recorded in mild drought stress (I₄₀) treated with Myco-Root + TiO₂ NPs. Based on the GC-MS and GC-FID analysis, 29 constituents were identified in peppermint EO, with the major constituents being menthol (38.99–52%), menthone (12.72–20.13%), 1,8-cineole (6.55–7.84%), and <i>neo</i>-menthol (3.14–4.52%), respectively. The maximum content of menthol, 1,8-cineole, and <i>neo</i>-menthol was obtained under mild drought stress (I₄₀) fertilized with Myco-Root + TiO₂ NPs. The results indicate that the integrative application of Myco-Root + TiO₂ NPs could be used as an alternative method of using chemical fertilizers in sustainable agricultural systems for improving the EO quantity and quality of peppermint grown under drought stress conditions.