Comparative Microscopic, Transcriptome and IAA Content Analyses Reveal the Stem Growth Variations in Two Cultivars <i>Ilex verticillata</i>
2023
Sini Qin | Siyi Fu | Ying Yang | Qiumin Sun | Jingqi Wang | Yanling Dong | Xinyi Gu | Tao Wang | Xiaoting Xie | Xiaorong Mo | Hangjin Jiang | Youxiang Yu | Jijun Yan | Jinfang Chu | Bingsong Zheng | Yi He
<i>Ilex verticillata</i> is not only an excellent ornamental tree species for courtyards, but it is also a popular bonsai tree. ‘Oosterwijk’ and ‘Red sprite’ are two varieties of <i>Ilex verticillata</i>. The former has a long stem with few branches, while the latter has a short stem. In order to explain the stem growth differences between the two cultivars ‘Oosterwijk’ and ‘Red sprite’, determination of the microstructure, transcriptome sequence and IAA content was carried out. The results showed that the xylem thickness, vessel area and vessel number of ‘Oosterwijk’ were larger than in ‘Red sprite’. In addition, our analysis revealed that the differentially expressed genes which were enriched in phenylpropanoid biosynthesis; phenylalanine metabolism and phenylalanine, tyrosine and tryptophan biosynthesis in the black and tan modules of the two varieties. We found that <i>AST</i>, <i>HCT</i> and <i>bHLH 94</i> may be key genes in the formation of shoot difference. Moreover, we found that the IAA content and auxin-related DEGs <i>GH3.6</i>, <i>GH3</i>, <i>ATRP5</i>, <i>IAA27</i>, <i>SAUR36-like</i>, <i>GH3.6-like</i> and <i>AIP 10A5-like</i> may play important roles in the formation of shoot differences. In summary, these results indicated that stem growth variations of ‘Oosterwijk’ and ‘Red sprite’ were associated with DEGs related to phenylpropanoid biosynthesis, phenylalanine metabolism and phenylalanine, tyrosine and tryptophan biosynthesis, as well as auxin content and DEGs related to the auxin signaling pathway.
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