Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach
2023
Camila A. Knecht | Maja Hinkel | Ines Mäusezahl | Anne-Kristin Kaster | Jaime Nivala | Jochen A. Müller
Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management of the global AMR crisis. Untreated and even treated wastewaters are important sources that release AMR into the environment. Methodologically, it is difficult to generate a comprehensive in situ profile of antibiotic resistance gene hosts. Here, we used epicPCR (emulsion, paired isolation, and concatenation PCR) as a cultivation-independent method to reveal the host profiles of the AMR indicator genes <i>intI1</i>, <i>sul1</i>, <i>sul2</i>, and <i>dfrA1</i> in two constructed wetlands treating municipal wastewater. Overall, the epicPCR analysis revealed a profile of AMR indicator gene hosts that is consistent with literature data from cultivation-based approaches. Most carriers of antibiotic resistance (AR) genes and likely of class 1 integrons belonged to the <i>Gammaproteobateria</i>, particularly the <i>Burkholderiaceae</i> and <i>Rhodocyclaceae</i> families, followed by members of the <i>Campylobacterota</i>, <i>Desulfobacterota</i>, and <i>Firmicutes</i>. The analysis also identified several novel hosts for the indicator genes widely distributed in the wetlands, including the genera <i>Legionella</i> and <i>Ralstonia</i>. Therefore, the application of epicPCR has produced an expanded insight into the in situ indicator gene host profile, while highlighting the role of the environment as a reservoir for AMR.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Directory of Open Access Journals