A cocktail PCR and DNA strip method for quick confirmation of multiple pathogenic factors in BSL3 stock cultures
2016
Ezaki, T. (Gifu University Graduate School of Medicine, Gifu (Japan). Regeneration and Advanced Medical Science) | Kanazawa, I. | Hayashi, S. | Hayashi, M. | Eldesoky, I. | Fukunaga, H.
Repositories of culture collections of Biosafety Level 3 (BSL3) pathogens are expected to provide information on the pathogenic factors present in stock bacteria. However, it is difficult to maintain valid pathogenic information on each of the different BLS3 pathogens because quality control of the stock strains generally has to be performed by a few staff members. We developed a common platform to differentiate six different PCR amplicons on a DNA strip. The six amplicons can be differentiated with the help of three position markers printed on the strip. With this method, the pathogenic factors in BSL3 pathogens can be determined within 40 min after cultured bacteria are picked up from a single colony.
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