Analysis of phenolics contents and phenolics biosynthesis-related PAL in Eucalyptus saligna leaves
2018
Inagaki, R. (The University of Tokyo (Japan). Graduate School of Agricultural and Life Sciences, Department of Biomaterial Sciences) | Terada, T. | Inoue, H. | Kamoda, S. | Samejima, M.
We investigated 80% methanol-soluble leaf extracts (predominantly phenolics) from 2-3 year-old Eucalyptus saligna (Es) leaves by colorimetric assay and HPLC. In the species E. saligna, a different line number was given depending on the production area. The flavanol content in Es 20483 leaf was clearly correlated to the leaf height from the ground, although other Es lines were not. Furthermore, PAL-like gene sequence was analyzed based on genomic DNA and mRNA extracted from leaves of three lines of Es. A total of 32 PAL-like gene sequences could be cloned from 3 Es lines, and among them, activity at the crude enzyme solution level was confirmed. Phylogenetic analysis showed that one clade was formed with 20483 PALs and 20483 strains had different evolutionary features from the other 2 lines. Within the Es species, there were differences in the mechanism of production and accumulation of phenolic components, and gene sequence depending on the lineage.
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