Morphological and cytochemical characteristics of non-phagocytic granulocytes from Japanese angelshark Squatina japonica
2018
Kondo, M. (National Fisheries University (Japan). Department of Applied Aquabiology) | Maekawa, K. | Yasumoto, S.
Two types of non-phagocytic granulocytes, type B and C granulocytes were observed in peripheral blood of Japanese angelshark Squatina japonica (Squatinidae, Squatiniformes, Squalimorphii, Elasmobranchii). Type B granulocyte (GB) had three types of granules (GBG-A, -B and -C) in the cytoplasm. The GBG-A was coarse, round and consisted of three layers (L0, chromophobic inner; L1, eosinophilic middle; L2, chromophobic outer). The GBG-B had similar shape, size and structure of GBG-A except for the staining character of middle layer (basophilic). The GBG-C was round, small and made up of two layers: Amphophilic inner (L0) and outer (L1) layers. The L1 of GBG-A and/or GBG-B showed positive reaction to toluidine blue (TB), Sudan black B (SBB), alpha-naphtyl acetate esterase (alpha-NAE), alpha-naphtyl butyrate esterase (alpha-NBE) and naphthol AS-D chloroacetate esterase (NASDCAE). On the other hand, in the GBG-C, SBB, acid phosphatase (AcP), alpha-NAE and NASDCAE were detected in L0, alpha-NBE in L1 and TB in both L0 and L1 Type C granulocyte (GC) had one type of granules (GCG) which show two-layer structure: Eosinophilic inner (L0) and outer (L1) layers. Several enzymes (AcP, alpha-NBE, NASDCAE) were detected in L0. Both types of non-phagocytic granulocytes lacked alkaline phosphatase, beta-glucuronidase and peroxidase.
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