The Pseudomonas viridiflava phylogroups in the P. syringae species complex are characterized by genetic variability and phenotypic plasticity of pathogenicity-related traits.
2014
Bartoli, Claudia | Berge, Odile | Monteil, Caroline | Guilbaud, Caroline | Balestra, Giorgio M. | Varvaro, Leonardo | Jones, Corbin D. | Dangl, Jeffery L. | Baltrus, David A | Sands, David, C. | Morris, Cindy E. | Università degli studi della Tuscia = Tuscia University [Viterbo] (UNITUS) | Unité de Pathologie Végétale (PV) ; Institut National de la Recherche Agronomique (INRA) | Department of Biology - Carolina Center for Genome Sciences ; Université de Caroline du Nord à Chapel Hill = University of North Carolina [Chapel Hill] (UNC-Chapel Hill) ; University of North Carolina System (UNC)-University of North Carolina System (UNC) | University of North Carolina System (UNC) | University of Arizona | Department Plant Sciences and Plant Pathology [Bozeman] ; Montana State University (MSU) | Tuscia University (Italy); Italian Minister of Agriculture and Food Polices (MIPAAF), Project ACTINIDIA - OIGA [247]; NIH [R01-GM066025]; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation [GBMF3030]; University of Arizona
We thank the staff of the INRAE Experimental facilities of the Plant Pathology research unit (IEPV, https://doi.org/10.15454/8DGF-QF70) for their involvement in field experiments
Mostrar más [+] Menos [-]International audience
Mostrar más [+] Menos [-]Inglés. As a species complex, Pseudomonas syringae exists in both agriculture and natural aquatic habitats. P. viridiflava, a member of this complex, has been reported to be phenotypically largely homogenous. We characterized strains from different habitats, selected based on their genetic similarity to previously described P. viridiflava strains. We revealed two distinct phylogroups and two different kinds of variability in phenotypic traits and genomic content. The strains exhibited phase variation in phenotypes including pathogenicity and soft rot on potato. We showed that the presence of two configurations of the Type III Secretion System [single (S-PAI) and tripartite (T-PAI) pathogenicity islands] are not correlated with pathogenicity or with the capacity to induce soft rot in contrast to previous reports. The presence/absence of the avrE effector gene was the only trait we found to be correlated with pathogenicity of P. viridiflava. Other Type III secretion effector genes were not correlated with pathogenicity. A genomic region resembling an exchangeable effector locus (EEL) was found in S-PAI strains, and a probable recombination between the two PAIs is described. The ensemble of the variability observed in these phylogroups of P. syringae likely contributes to their adaptability to alternating opportunities for pathogenicity or saprophytic survival.
Mostrar más [+] Menos [-]Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique