OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions
2020
Lee, Sichul | Park, Joonheum | Lee, Jinwon | Shin, Dongjin | Marmagne, Anne | Lim, Pyung Ok | Masclaux-Daubresse, Céline | An, Gynheung | Nam, Hong Gil | Center for Plant Aging Research ; Institute for Basic Science | Rural Development Administration ; National Institute of Horticultural and Herbal Science (NIHHS) | Institut Jean-Pierre Bourgin - Sciences du végétal (IJPB) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Daegu Gyeongbuk Institute of Science and Technology (DGIST) | Kyung Hee University (KHU) | Institute for Basic Science (IBS-R013-D1) | PHC STAR no 34300ZK and 2017K1A3A1A21013795 | ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010)
International audience
Mostrar más [+] Menos [-]Inglés. Abstract Nitrogen (N) is a major limiting factor affecting crop yield in unfertilized soil. Thus, cultivars with a high N use efficiency (NUE) and good grain protein content (GPC) are needed to fulfill the growing food demand and to reduce environmental burden. This is especially true for rice (Oryza sativa L.) that is cultivated with a high input of N fertilizer and is a primary staple food crop for more than half of the global population. Here, we report that rice asparagine synthetase 1 (OsASN1) is required for grain yield and grain protein contents under both N-sufficient (conventional paddy fields) and N-limiting conditions from analyses of knockout mutant plants. In addition, we show that overexpression (OX) of OsASN1 results in better nitrogen uptake and assimilation, and increased tolerance to N limitation at the seedling stage. Under field conditions, the OsASN1 OX rice plants produced grains with increased N and protein contents without yield reduction compared to wild-type (WT) rice. Under N-limited conditions, the OX plants displayed increased grain yield and protein content with enhanced photosynthetic activity compared to WT rice. Thus, OsASN1 can be an effective target gene for the development of rice cultivars with higher grain protein content, NUE, and grain yield under N-limiting conditions.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique