Two-and Three-dimensional Cell Structures Govern Epidermal Growth Factor Survival Function in Human Bladder Carcinoma Cell Lines
1997
Dangles, Virginie | Féménia, Françoise | Laine-Prade, Veronique | Berthelemy, Madeleine | Le Rhun, Danielle | Poupon, Marie France | Levy, Daniel | Schwartz-Cornil, Isabelle | Faculté des Sciences Pharmaceutiques et Biologiques | Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques ; Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | École nationale vétérinaire d'Alfort (ENVA) | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) ; Institut National de la Recherche Agronomique (INRA)
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Mostrar más [+] Menos [-]Inglés. Human bladder carcinomas often express high levels of the epidermal growth factor (EGF) receptor. In three human bladder carcinoma cell lines (OBR, T24, and 647V), we show that two EGF receptor ligands, namely EGF and transforming growth factor alpha, enhanced the apoptosis due to serum starvation on cells cultured as monolayers. Conversely, EGF and transforming growth factor alpha prevented apoptosis when the same serum-starved cells were cultured as three-dimensional spheroids. Both stimulation and inhibition of apoptosis by EGF were associated with p21 WAF1/CIP1 overexpression. In 647V spheroids, EGF protection against radiation-induced apoptosis was negated by genistein and tyrphostin AG1478, suggesting that blockade of the EGF signal transduction in patients with bladder cancer may improve the radiotherapy efficacy
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