EU-OSTID: A collection of transposon insertional mutants for functional genomics in rice
2005
van Enckevort, Ellen | Droc, Gaëtan | Piffanelli, Pietro | Greco, Raffaella | Gagneur, Cyril | Weber, Christelle | Gonzalez, Victor M. | Cabot, Pere | Fornara, Fabio | Berri, Stefano | Miró, Berta | Lan, Ping | Capell, Teresa | Puigdomenech, Pere | Ouwerkerk, Pieter B.F. | Meijer, Annemarie H. | Pe, Enrico | Colombo, Lucia | Christou, Paul | Guiderdoni, Emmanuel | Pereira, Andy | Plant Research International (PRI) ; Wageningen University and Research [Wageningen] (WUR) | Polymorphismes d'intérêt agronomique (UMR PIA) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA) | Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | Dipartimento di Biologia ; Università degli Studi di Milano = University of Milan (UNIMI)-Sez. Zoologia Scienze Naturali | Università degli Studi di Milano = University of Milan (UNIMI) | Fraunhofer (Fraunhofer-Gesellschaft) | Universiteit Leiden = Leiden University
author for correspondence; e-mail [email protected]
Mostrar más [+] Menos [-]International audience
Mostrar más [+] Menos [-]Inglés. A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique