Imaging InIC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
2013
Kuehbacher, Andreas | Gouin, Edith | Cossart, Pascale | Pizarro-Cerdá, Javier | Interactions Bactéries-Cellules (UIBC) ; Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) | USC2020 ; Inst Natl Rech Agron | Pasteur Institute ; Institut National de la Sante et de la Recherche Medicale ; Institut National de la Recherche Agronomique ; ERC 233348 ; Agence Nationale de la Recherche ; Louis-Jeantet Foundation ; Fondation Le Roch Les Mousquetaires ;Pasteur-Paris University International Doctoral Program/Institut Carnot Maladies Infectieuses
International audience
Mostrar más [+] Menos [-]Inglés. Bacterial intracellular pathogens can be conceived as molecular tools to dissect cellular signaling cascades due to their capacity to exquisitely manipulate and subvert cell functions which are required for the infection of host target tissues. Among these bacterial pathogens, Listeria monocytogenes is a Gram positive microorganism that has been used as a paradigm for intracellular parasitism in the characterization of cellular immune responses, and which has played instrumental roles in the discovery of molecular pathways controlling cytoskeletal and membrane trafficking dynamics. In this article, we describe a robust microscopical assay for the detection of late cellular infection stages of L. monocytogenes based on the fluorescent labeling of InIC, a secreted bacterial protein which accumulates in the cytoplasm of infected cells; this assay can be coupled to automated high-throughput small interfering RNA screens in order to characterize cellular signaling pathways involved in the up-or down-regulation of infection.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique