The art of growing plants for experimental purposes: a practical guide for the plant biologist
2012
Poorter, Hendrik | Fiorani, Fabio | Planck, Max | Schurr, Uli | Finck, Alex | Gibon, Yves, Y. | Usadel, Björn | Munns, Rana | Atkin, Owen K. | Tardieu, Francois, F. | Pons, Thijs L. | Forschungszentrum Jülich GmbH | Centre de recherche de Jülich | Jülich Research Centre (FZJ) ; Helmholtz-Gemeinschaft = Helmholtz Association | Max Planck Institute of Molecular Plant Physiology (MPI-MP) ; Max-Planck-Gesellschaft | Biologie du fruit et pathologie (BFP) ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) | RWTH Aachen University = Rheinisch-Westfälische Technische Hochschule Aachen (RWTH Aachen) | The University of Western Australia (UWA) | Australian National University (ANU) | Écophysiologie des Plantes sous Stress environnementaux (LEPSE) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Universiteit Utrecht / Utrecht University [Utrecht] | European Community [FP7-244374] | European Project: 244374,EC:FP7:KBBE,FP7-KBBE-2009-3,DROPS(2010)
International audience
Mostrar más [+] Menos [-]Inglés. Every year thousands of experiments are conducted using plants grown under more-or-less controlled environmental conditions. The aim of many such experiments is to compare the phenotype of different species or genotypes in a specific environment, or to study plant performance under a range of suboptimal conditions. Our paper aims to bring together the minimum knowledge necessary for a plant biologist to set up such experiments and apply the environmental conditions that are appropriate to answer the questions of interest. We first focus on the basic choices that have to be made with regard to the experimental setup (e.g. where are the plants grown; what rooting medium; what pot size). Second, we present practical considerations concerning the number of plants that have to be analysed considering the variability in plant material and the required precision. Third, we discuss eight of the most important environmental factors for plant growth (light quantity, light quality, CO2, nutrients, air humidity, water, temperature and salinity); what critical issues should be taken into account to ensure proper growth conditions in controlled environments and which specific aspects need attention if plants are challenged with a certain a-biotic stress factor. Finally, we propose a simple checklist that could be used for tracking and reporting experimental conditions.
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Información bibliográfica
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