Mapping with a few plants: Using selective mapping for microsatellite saturation of the prunus reference map
2005
Howad, Werner | Yamamoto, Toshiya | Dirlewanger, Elisabeth | Testolin, R. | Cosson, Patrick | Cipriani, Guido | Monforte, Antonio J. | Georgi, L. L. | Abbott, Albert G. | Arús, Pere | Ministerio de Ciencia y Tecnología (España)
The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond × peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.
Mostrar más [+] Menos [-]Funds for this research have been partly provided by projects FIT-010000-2001-112 and AGL2003-04691 of the Spanish Ministry of Science and Technology.
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