Effects of parental diets supplemented with different lipid sources on Octopus maya embryo and hatching quality

The present research showed, for the 1st time, the effect of formulated diets for broodstock of Octopus maya. Reproductive response of O. maya females fed prepared diets and a control diet (crab) was evaluated according to reproductive performance and hatchling quality. Females were fed three formulated most diets based on: 1) crab and squid (CS), 2) crab, squid and fish heads (CSF) and 3) crab, squid and SELCO ® (CSS). Fresh crab (Callinectes sapidus) (Diet 4, C) was used as a control diet. A total of 10 females were used for each experimental diet (n = 40). Females were fed 5% body weight d− 1 until spawning. The feeding period of females was 50 days on average to natural spawning (p < 0.05). Eggs laid by each female were placed in separate incubators with similar temperature, light and water quality, which were thoroughly controlled. Total egg number, egg batches, fecundity, spawning, newly hatched weight and survival after 10 days unfed were determined for each spawn. Egg and hatchling biochemical composition, as well as soluble protein content from the perivitelline was determined for each spawn. Embryonic development of eggs was also studied. No differences in reproductive performance were observed between the four diets. Contrary, PCO analysis showed marked differences on fatty acid composition of the yolk and hatchlings. Palmitic acid (16:0) and arachidonic acid (20:4n6) were the main contributors for the first coordinate (eigenvalues, λ of 0.54 and 0.55 for embryo and hatchlings yolk, respectively). Females fed the control diet (C) produced hatchling with higher weight (p < 0.05). Diet 3 (CSS) delivered the largest hatchlings (p < 0.05) among the prepared diets. There were no differences (p > 0.05) in soluble protein content of the perivitelline or newly hatched survival after 10 days unfed. Morphometric differences on 40 days old embryos were associated with females (p < 0.05) but not with each diet (p > 0.05). Results obtained indicate that O. maya female's present marked adaptation to the diets ingested prior to egg laying, without compromising reproductive performance.

CONACYT and the Inter-American Council for agriculture are appreciated by Fernando Tercero scholarship for studies of masters. This research was partially financed by the project PAPIIT IT200713 from the DGAPA-UNAM to Pedro Gallardo and CONACYT-CB201001 project No. 150810 to Carlos Rosas. Thanks also to PASPA — UNAM through a scholarship program to Pedro Gallardo for sabbatical stay at the ULPGC de Gran Canaria, Spain. We thank Korynthia López from the “Unidad de Química-Sisal” for guidance and advice, as well as Stephanie Bernal Pérez for her help in biochemical determinations.

Peer reviewed