Optimization of micropropagation protocols in some wooody plants using meta-topolin
2021
San José, M. Carmen | Cernadas, María José | Janeiro, L.V.
The type and concentration of growth regulators used in micropropagation vary widely, depending on the species that are being studied and they have to be optimized depending on growth parameters: multiplication rates, shoot quality, rooting, and acclimatization. Cytokinins are an essential class of growth regulators that along with auxins, control numerous physiological and plant development processes. The choice of cytokinin is one of the most critical factors in the development of protocols for the in vitro culture of plants. N6 -benzyladenine (BA) is currently one of the most widely used cytokinins, due to its efficacy and relatively low cost. However, the use of this cytokinin in micropropagation procedures can produce physiological disorders such as hyperhydricity, apical necrosis, senescence, rooting inhibition, andacclimatization issues. These have necessitated search for alternatives to BA in order to maintain reasonable proliferation rates and acceptable plant quality. The discovery of a new group of aromatic cytokinins, the topolins, has led to new possibilities for their use in the micropropagation of numerous species. These compounds can be considered an alternative to N6 -benzyladenine (BA), trans-zeatin (tZ), N6 -isopentenyladenine (iP), and kinetin (KIN). In the last few years, the use of meta-topolin (mT) and its derivatives has increased rapidly, especially in initiating new cultures, optimizing protocols, and inhibiting the negative features of some other cytokinins. This chapter is a review of the role of meta-topolin in the optimizing micropropagation in woody plant tissue cultures.
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