Multi‐generational responses of a marine polychaete to a rapid change in seawater pCO2

Data archiving statement: Life‐history, metabolic and water chemistry data have been deposited in the British Oceanographic Data Centre (http://www.bodc.ac.uk, doi:10.5285/22b54764‐2448‐1318‐e053‐6c86abc01ae1).

Little is known of the capacity that marine metazoans have to evolve under rapid pCO2 changes. Consequently, we reared a marine polychaete, Ophryotrocha labronica, previously cultured for approximately 33 generations under a low/variable pH regime, under elevated and low pCO2 for six generations. The strain used was found to be tolerant to elevated pCO2 conditions. In generations F1 and F2 females’ fecundity was significantly lower in the low pCO2 treatment. However, from generation F3 onwards there were no differences between pCO2 treatments, indicating that trans‐generational effects enabled the restoration and maintenance of reproductive output. Whilst the initial fitness recovery was likely driven by trans‐generational plasticity (TGP), the results from reciprocal transplant assays, performed using F7 individuals, made it difficult to disentangle between whether TGP had persisted across multiple generations, or if evolutionary adaptation had occurred. Nonetheless, both are important mechanisms for persistence under climate change. Overall, our study highlights the importance of multi‐generational experiments in more accurately determining marine metazoans’ responses to changes in pCO2, and strengthens the case for exploring their use in conservation, by creating specific pCO2 tolerant strains of keystone ecosystem species.

This work was supported by a NERC‐Defra‐DEC UKOA Research Programme grant NE/H017127/1 to J.I.S. and P.C. A.R.R. was supported by a Junta de Andalucía (Spanish Regional Government) grant RNM‐3924. P.C. is supported by a NSERC Discovery Program grant and G.M.N. is supported by a EU Marie Curie‐Skłodowska Research Fellowship (EVOLMARIN‐GA 659359).

Peer reviewed