Genesis, Evolution, and Genetic Diversity of the Hexaploid, Narrow Endemic Centaurea tentudaica

Within the genus Centaurea L., polyploidy is very common, and it is believed that, as to all angiosperms, it was key in the history of its diversification and evolution. Centaurea tentudaica is a hexaploid from subsect. Chamaecyanus of unknown origin. In this study, we examined the possible autopolyploid or allopolyploid origin using allozymes and sequences of three molecular markers: nuclear-ribosomic region ETS, and low-copy genes AGT1 and PgiC. We also included three species geographically and morphologically close to C. tentudaica: C. amblensis, C. galianoi, and C. ornata. Neighbor-Net and Bayesian analyses show a close relationship between C. amblensis and C. tentudaica and no relationship to any of the other species, which suggest that C. tentudaica is an autopolyploid of C. amblensis. Allozyme banding pattern also supports the autopolyploidy hypothesis and shows high levels of genetic diversity in the polyploid, which could suggest multiple origins by recurrent crosses of tetraploid and diploid cytotypes of C. amblensis. Environmental niche modeling was used to analyze the distribution of the possible parental species during the present, Last Glacial Maximum (LGM), Last Interglacial Period (LIG), and Penultimate Glacial Maximum (PGM) environmental conditions. Supporting the molecular suggestions that C. tentudaica originated from C. amblensis, environmental niche modeling confirms that past distribution of C. amblensis overlapped with the distribution of C. tentudaica.

This research was funded by the Catalan government (“Ajuts a grups consolidats” 2017-SGR1116).

Abstract 1. Introduction 2. Materials and Methods 2.1. Sampling Strategy and Markers 2.2. DNA Amplification, Cloning, and Sequencing 2.3. Sequence Alignment and Phylogenetic Analyses 2.4. Allozyme Extraction and Analyses 2.5. Ecological Niche Modeling 3. Results 3.1. ETS Region 3.2. Low-copy Genes AGT1 and PgiC 3.3. Allozymes 3.4. Ecological Niche Modeling 4. Discussion 4.1. Relative Utility of ETS, AGT1, and PgiC in Disentangling the Origin of Centaurea tentudaica 4.2. The Origin of Centaurea tentudaica 5. Conclusions