Nodulation of <em>Aeschynomene afraspera</em> and <em>A. indica</em> by photosynthetic <em>Bradyrhizobium</em> sp. strain ORS285: the nod-dependent versus the nod-independent symbiotic interaction
2011
Bonaldi, Katia | Gargani, Daniel | Prin, Yves | Fardoux, Joel | Gully, Djamel | Nouwen, Nico | Goormachtig, Sofie | Giraud, Eric | Institut de Recherche pour le Développement (IRD) | Université Montpellier 2 - Sciences et Techniques (UM2) | Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Department of plant systems biology ; Flanders Institute for Biotechnology | Department of Plant Biotechnology and Genetics ; Universiteit Gent = Ghent University = Université de Gand (UGENT) | French national research agency [ANR-NEWNOD-2006-BLAN-0095, ANR-SESAM-2010-BLAN-170801]; bilateral Franco-Belgium Egide program [18074NJ]; Ministere de l'Education Nationale, de l'Enseignement Superieur et de la Recherche | ANR-06-BLAN-0095,NewNod,Identification of new molecular determinants involved in the early steps of symbiotic interactions between plants and bacteria(2006)
International audience
Mostrar más [+] Menos [-]Inglés. Here, we present a comparative analysis of the nodulation processes of Aeschynomene afraspera and A. indica that differ in their requirement for Nod factors (NF) to initiate symbiosis with photosynthetic bradyrhizobia. The infection process and nodule organogenesis was examined using the green fluorescent protein labeled Bradyrhizohium sp. strain ORS285 able to nodulate both species. In A. indica, when the NF-independent strategy is used, bacteria penetrated the root intercellularly between axillary root hairs and invaded the subepidermal cortical cells by invagination of the host cell wall. Whereas the first infected cortical cells collapsed, the infected ones immediately beneath kept their integrity and divided repeatedly to form the nodule. In A. afraspera, when the NF-dependent strategy is used, bacteria entered the plant through epidermal fissures generated by the emergence of lateral roots and spread deeper intercellularly in the root cortex, infecting some cortical cells during their progression. Whereas the infected cells of the lower cortical layers divided rapidly to form the nodule, the infected cells of the upper layers gave rise to an outgrowth in which the bacteria remained enclosed in large tubular structures. Together, two distinct modes of infection and nodule organogenesis coexist in Aeschynomene legumes, each displaying original features.
Mostrar más [+] Menos [-]Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique