Detection of vancomycin resistance genes in enterococcal isolates of animal origin by multiplex PCR
2002
Mac, K. | Wichmann-Schauer, H. | Peters, J. | Ellerbroek, L.
Alemán. 207 enterococcal isolates of animal and food origin were examined for the existence of vancomycin resistance genes (vanA, vanB, vanC1, vanC2) by multiplex-PCR. For 5 strains the vanA-gene could be detected clearly. The vanB-gene could not be found in any of the examined strains. In subsequent single PCR examinations 135 vanC-positive strains were identified as either vanC1- or vanC2-isolates whereas 16 vanC-positive probes were obviously mixed cultures. For 5 enterococcal strains isolated from pigs the multiplex FOR led first to false-negative results. By single FOR examinations the vanC1-gene could be detected in these strains. According to multiplex- and single FOR examinations 46 strains were negative for any examined gene. The multiplex FOR is characterised by its time-and material saving advantages and is therefore recommended for the routine use for the detection of vancomycin genes in enterococcal strains. For the differentiation of vanC1- and vanC2-genes and the clarification of negative results single-PCR examinations should be carried out
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ISSN 0003-925XEste registro bibliográfico ha sido proporcionado por German Federal Institute for Risk Assessment