Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells
2007
Andersson, Maria | Hakulinen, Pasi | Honkalampi-Hamalainen, Ulla | Hoornstra, Douwe | Lhuguenot, Jean-Claude | Mäki-Paakkanen, Jorma | Savolainen, Martti | Séverin, Isabelle | Stammati, Anna-Laura | Turco, Laura | Weber, Assi | von Wright, Atte | Zucco, Flavia | Salkinoja-Salonen, Mirja | Department of Applied Chemistry and Microbiology [Helsinki] ; Helsingin yliopisto = Helsingfors universitet = University of Helsinki | National Public Health Institute [Finland] (THL) | University of Kuopio, Institute of Applied Biotechnology | Laboratoire Toxicologie Alimentaire ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA) | KCL Science and Consulting ; KCL Science and Consulting | Department of Environmental and Primary Prevention ; Istituto Superiore di Sanità = National Institute of Health (ISS) | Istituto di Neurobiologia e Medicina Molecolare ; National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR)
International audience
Mostrar más [+] Menos [-]Inglés. Some strains of the endospore-forming bacterium Bacillus cereus produce a heat-stable ionophoric peptide, cereulide, of high human toxicity. We assessed cell toxicity of cereulide by measuring the toxicities of crude extracts of cereulide producing and non-producing strains of B. cereus, and of pure cereulide, using cells of human, animal and bacterial origins. Hepatic cell lines and boar sperm, with cytotoxicity and sperm motility, respectively, as the end points, were inhibited by <= 1 nM of cereulide present as B. cereus extract. RNA synthesis and cell proliferation in HepG2 cells was inhibited by 2 nM of cereulide. These toxic effects were explainable by the action of cereulide as a high-affinity mobile K+ carrier. Exposure to cereulide containing extracts of B. cereus caused neither activation of CYPlA1 nor genotoxicity (comet assay, micronucleus test) at concentrations below those that were cytotoxic (0.6 nM cereulide). Sahnonella typhimurium reverse mutation (Ames) test was negative. Exposure of Vibrio fischeri to extracts of B. cereus caused stimulated luminescence up to 600%, independent on the presence of cereulide, but purified cereulide inhibited the luminescence with an IC50% (30min) of 170nM. Thus the luminescence-stimulating B. cereus substance(s) masked the toxicitv of cereulide in B. cereus extracts to V fischeri. (c) 2006 Elsevier Ltd. All rights reserved.
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