Relative Saccharification of Sawdust Materials at Different Incubation pH-values
2024
N. A. Ndukwe, J. B. M. Seeletse and J. P. H. van Wyk
The uncontrolled production of waste is a daily phenomenon that is experienced by most global communities, and the situation worsens due to the lack of effective waste management procedures. Solid waste such as sawdust is primarily produced by the forestry industry and although it is utilized by certain countries as briquettes to make fire or as an absorbent to clean fluid spillage as well as a component of ceilings, most of the sawdust along the Lagos Lagoon in Nigeria is left unattended as waste, contributing to environmental pollution. Cellulose, composed of glucose units is a structural component of sawdust and when saccharified the resulting glucose can be fermented into renewable substances such as bio-ethanol. The cellulose degradation process can be performed with a cellulase enzyme such as available in the fungus Aspergillus niger and during the current investigation, this enzyme system was used to bio-convert the cellulose component of sawdust from ten different trees along the Lagoon into glucose. To increase the cellulase action all sawdust materials were delignified before cellulase action with the main aim of determining the optimum pH value for maximum degradation of the various sawdust materials. The pH-related saccharification profile of each type of sawdust was constructed as well as the relative percentage of saccharification and it was concluded that all the materials were optimum degraded at acidic pH-values which varied between pH 5.0 and pH 6.0 that are like optimum pH-values reported for the other types of cellulose materials.
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