Potential of Fiber and Probiotics to Fight Against the Effects of PhIP + DSS-Induced Carcinogenic Process of the Large Intestine

We determined the in vivo counteracting effect of fiber and probiotic supplementation on colonic mucosal damage and alterations in gut microbiota caused by 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) and sodium dextran sulfate (DSS). Male Fischer-344 rats were randomly divided into 4 groups: control (standard diet), PhIP + DSS group (standard diet + PhIP + DSS), fiber (fiber diet + PhIP + DSS), and probiotic (probiotic diet + PhIP + DSS). The intake of PhIP + DSS for 3 weeks induced colonic mucosal erosion, crypt loss, and inflammation, and the distal colon was more severely damaged. Fiber alleviated colonic mucosal damage by reducing crypt loss and inflammation, while the probiotic increased colon length. The intake of PhIP + DSS increased the fecal relative abundance of Clostridia UCG014 along the intervention, in contrast to the lower abundances of these taxa found after PhIP + DSS administration in the rats supplemented with probiotics or fiber. Fiber supplementation mitigated the histological damage caused by PhIP + DSS shifting the gut microbiota toward a reduction of pro-inflammatory taxa.

This work was funded by Project RTI2018−098 288−B-I00(acronym MIXED), financed by MCIN/AEI/10.13 039/501100011033/FEDER, “Una manera de hacer Europa”; byProject AYUD/2021/50 981, financed with regional fundsfrom the Plan Regional de Investigación, Principality ofAsturias; and by the Grant PID2022−140410OB-I00, fundedby MCIN/AEI/10.13 039/501100011033/FEDER “A way ofmaking Europe”. AZ is a recipient of a predoctoral contract(Severo Ochoa PA-23-BP22−034) funded by the PlanRegional de Investigación from the Principality of Asturias

Peer reviewed