Glycosaminoglycans inhibit the adherence and the spreading of osteoclasts and their precursors: role in osteoclastogenesis and bone resorption. | Glycosaminoglycans inhibit the adherence and the spreading of osteoclasts and their precursors: role in osteoclastogenesis and bone resorption.: glycosaminoglycans and osteoclastogenesis
2011
Baud'Huin, Marc | Ruiz-Velasco, Carmen | Jego, Gaëtan | Charrier, Céline | Gasiunas, Nijole | Gallagher, John | Maillasson, Mike | Naggi, Annamaria | Padrines, Marc | Redini, Françoise | Duplomb, Laurence | Heymann, Dominique | Physiopathologie des Adaptations Nutritionnelles (PhAN) ; Institut National de la Recherche Agronomique (INRA)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE) ; Université de Nantes (UN)-Université de Nantes (UN) | Centre de Recherche en Cancérologie Nantes-Angers (CRCNA) ; Université d'Angers (UA)-Centre Hospitalier Universitaire d'Angers (CHU Angers) ; PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE) ; Université de Nantes (UN)-Université de Nantes (UN) | Paterson Institute for Cancer Research ; University of Manchester [Manchester] | Institute for Chemical and Biochemical Research G. Ronzoni ; Institute for Chemical and Biochemical Research G. Ronzoni | Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes) | This work was supported by the Région des Pays de la Loire [Program entitled "Ciblage Moléculaire et Applications Thérapeutique" (CIMATH)] and by the ANR 2007 INSERM Pathophysiology of Human Diseases project N° R07196NS. Marc Baud'huin received a fellowship from the Région des Pays de la Loire.
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Mostrar más [+] Menos [-]Inglés. The bone microenvironment (e.g. glycosaminoglycans (GAGs), growth factors) plays a major role in bone resorption, especially in the formation of osteoclasts which differentiate from the hematopoietic lineage in the presence of RANKL. Previous studies revealed that GAGs may influence osteoclastogenesis, but data are very controversial, some studies showing an inhibitory effect of GAGs on osteoclastic differentiation whereas others demonstrated a stimulatory effect. To clarify their activities, we investigated the effect of 5 families of GAGs in three different models of human/mouse osteoclastogenesis. The present data revealed that heparin inhibited osteoclastogenesis in these three models, which was confirmed by a decrease in mRNA expression of osteoclastic markers and by an inhibition of the bone resorption capacity. We also demonstrated in RAW 264.7 cells that other families of GAGs different from heparin inhibited RANKL-induced osteoclastogenesis, and that this inhibition was dependent on the length and the level of sulfation of GAGs. In the present work, heparin did not bind to RANKL and did not modulate RANKL signaling. Heparin acted at 2 distinct steps of osteoclastogenesis from human CD14(+) cells: first, heparin strongly decreased the adherence of osteoclast precursors, and secondly inhibited osteoclasts to spread and to be active. Furthermore, the second action of heparin was reversible as the removal of heparin at the end of the culture time allowed the condensed cells to spread out and showed the formation of morphological active osteoclasts. The present work clearly evidences that GAGs inhibit osteoclastogenesis in vitro and strengthens the therapeutic interest of defined GAGs in osteolytic diseases.
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