Cytoplasmic Genome Prediction in Cucumber (Cucumis sativus L.) Hybrid Variety Breeding
2022
Akar, Leyla Öztürk | Gülsen, Osman | Zengin, Sinan | Vural, G. Elif
Breeding studies in Cucurbitaceae species take a long time. It has become necessary to shorten the time and supporttraditional breeding methods with modern biotechnological methods to get qualified domestic cucumber varieties.Cytoplasmic genome prediction within the scope of molecular-based breeding is a very important application. To increaseheterosis in test crosses, reciprocal ‘double way’ crosses can be made as well as single crosses. Cytoplasmic organelles‘plastid and mitochondria’ are considered to be different from each other between individuals and reciprocal crosses aremade based on this idea. However it significantly increases the labor. In this study, 4 plastid genome regions (rbcL, psbtrnS, trnHK, trnSt) located within non-conserved regions therefore expected to be variable of 50 donor genotypes weresequenced, analyzed and their cytoplasmic genome prediction was estimated. A total of 6300 bp including four plastidregions indicated no polyfmorphism and all sequences were identical among the 50 donor genotypes analyzed. This mayimply no cytoplasmic organelle variation. In conclusion, reciprocal crosses were excluded from our breeding studies. Socytoplasmic genome prediction can provide rapidity and savings in breeding by eliminating unnecessary reciprocal testcrosses.
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