Genetic Diversity of Food-Isolated Salmonella Strains through Pulsed Field Gel Electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
2013
Fendri, Imen | Ben Hassena, Amal | Grosset, Noel | Barkallah, Mohamed | Khannous, Lamia | Chuat, Victoria | Gautier, Michel | Gdoura, Radhouane | Unité de Recherche Toxicologie, Microbiologie Environnementale et Santé ; جامعة صفاقس - Université de Sfax - University of Sfax | Unité de Microbiologie (MIC) ; Institut National de la Recherche Agronomique (INRA) | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Ministere de l'enseignement superieur et de la recherche scientifique
All over the world, the incidence of Salmonella spp contamination on different food sources like broilers, clams and cow milk has increased rapidly in recent years. The multifaceted properties of Salomnella serovars allow the microorganism to grow and multiply in various food matrices, even under adverse conditions. Therefore, methods are needed to detect and trace this pathogen along the entire food supply network. In the present work, PFGE and ERIC-PCR were used to subtype 45 Salmonella isolates belonging to different serovars and derived from different food origins. Among these isolates, S.Enteritidis and S. Kentucky were found to be the most predominant serovars. The Discrimination Index obtained by ERICPCR (0.85) was slightly below the acceptable confidence value. The best discriminatory ability was observed when PFGE typing method was used alone (DI = 0.94) or combined with ERIC-PCR (DI = 0.93). A wide variety of profiles was observed between the different serovars using PFGE or/and ERIC-PCR. This diversity is particularly important when the sample origins are varied and even within the same sampling origin.
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