COMPARISON BETWEEN COMPETITIVE ELISA AND ROSE-BENGAL TESTS IN DETECTION OF BRUCELLA ANTIBODIES IN BUFFALO SERA IN MOSUL CITY, IRAQ
2009
O. Kh.Al-Hankawe | M.O. Abdul-Majeed | O.M.Al-Iraqi | M.I.Al-Farwachi
The present study was conducted to compare C-ELISA and Rose-Bengal tests in detection of brucella antibodies in buffalo sera in Mosul city, using 132 adult females of indigenous buffaloes from May 2007 to May 2008. Seroprevalence was 50.8% using C-ELISA, and 28.8% when RBT have been used. The data obtained were analyzed statistically to identify the agreement between C-ELISA and RBT using Kappa value. Kappa index was (0.353), which indicates less agreement between the two tests. There were false-negative results of RBT in 36 samples out of 67 samples positive to C-ELISA, and 7 samples recorded as false-positive with RBT out of 65 samples negative to C-ELISA.
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