EFFECT OF NITRIC OXIDE DONOR SODIUM NITROPRUSSIDEON SPERM VOLUME OF DILUTED BULL SEMEN
2012
Eman Rassol abd Alshaty
Study the effect of nitric oxide donor on sperms membrane integrity and volume and their relationship with viability and sperm motility. This study was done usingtwo groupseach one contained 10 samples first one exposed to eight different gradient of hypotonic solutions containing Sodium nitroprusside (SNP)and second 10 samples diluted with gradient hypotonic solutions without Sodium nitroprusside,Bull semen tris dilution treated with Sodium nitroprussidein that protected the sperm fromosmocellular changes stress. The results showed tolerance sperm to gradient hypotonic solution in sperm swelling and classic spermatocrit marked significance by the relative volume shift volumetric data. In addition the SNP had sperm protection to osmolarity tested and give improvement viability and sperm motility. Hypotonic media tonicity that may be attributed to direct liberation of Nitric oxide that produced vital regulation of Na-K ATPase and Calcium channels of sperm membrane
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