DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI ISOLATED FROM FROZEN MEAT IN BASRAH MARKET
2016
Basil Abbas | Aula Alghanim
This study was aimed to detect the presence of Escherichia coli in frozen meat. A total of200 samples were collected from Basrah markets in the period extending from September2015 to March 2016. These samples were composed of 50 frozen fish samples, 50 frozenburger samples, 50 frozen chicken samples and(50) worker's hands swabs. Differenttechniques were used in this study to evaluate the presence of Escherichia coli whichcontaminate the frozen meat, these techniques included the traditional bacteriological assays,commercial identification kit (API 20 E) and molecular techniques (PCR). Results of thesetechniques indicated 25 (12.5%) samples were positive to Escherichia coli , as identified byAPI 20 E system. The results of 25 isolates of Escherichia coli which confirmed by PCR,These isolates were subjected to PCR [sta gene, stb gene, lt gene and uspA gene]. The resultsPCR confirmed only 16 of these isolates contain sta gene and 5 of these isolates contain uspAgene ,While isolates do not contain the gene stb or lt genes.
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