INFLUENCE OF GRADED LEVELS OF NIGELLA SATIVA SEEDS AND ROSMARINUS OFFICINALIS LEAVES ON IN VITRO RUMEN FERMENTATION AND APPARENT BIOHYDROGENATION
2018
Kifah Jumaah Odhaib
The results in present study are illustrated that the effects of different levels of NSseeds and RO leaves on in vitro gas production, rumen fermentation, fatty acidscomposition and the apparent biohydrogenation of oleic, linoleic and linolenic acidsusing rumen liquor from Dorper lambs. The NS seeds and RO leaves weresupplemented at the rate of 0, 0.5, 1, 1.5 and 2% (w/w) DM of basal substrate [60%forage (urea treated rice straw) and 40% concentrate] and incubated for 24 h at 39°C.Substrates containing RO and NS had greater (P<0.05) gas production than the controlsubstrates. The volume of gas produced increased as the levels of RO and NSincreased up to 1.5% and decreased afterwards. Supplementation of RO and NS did not affect (P>0.05) in vitro dry matter digestibility, in vitro organic matterdigestibility, rumen pH, CH4 and NH3-N, total volatile fatty acids (VFA) and themolar proportion of acetate, propionate and butyrate. The RO supplements reduced theruminal concentration of C18:0 and increased the ruminal concentration of C18:1n-9in a dose dependent manner. The supplementation of RO leaves reduced (P<0.05) theapparent biohydrogenation of C18:1n-9 but had no effect (P>0.05) on the apparentbiohydrogenation of C18:2n-6 and C18:3n-3.
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