Histological and Biochemical Investigation of Hepato-protective Role of Vitamin B12 in Male Wistar Rats Intoxicated by Diazinon
2022
Aseel Hameed
In order to estimate the hepato-protective activity of Vit.B12 in diazinon hepatotoxic effect, the current study was performed on twenty-four male adult Wistar rats. They’d divided into four equal groups for 30 days: group A (control group) served as control; group B (Dzn group) administered an oral daily dose of 1/10 LD50 (3.8mg/ kg.bw) from diazinon; group C (Dzn + Vit.B12 group) administered an oral daily dose of 1/10 LD50 (3.8 mg / kg bw) from diazinon, in addition to the systemic intramuscular doses of (4 mg/kg) from Vit.B12 in daily basis; group D (Vit.B12 group) administered daily systemic intramuscular doses of (4 mg/kg) Vit.B12 only. The results showed that group C had a significant improvement in the antioxidant indicators compared with group B in which the CAT, GSH, and SOD serum activities showed significant increasing (P≤0.05) in group C, in addition, it revealed a significant decreasing (P≤0.05) in the values of MDA and peroxynitrite comparing to the same levels of the group B. The histological results of the liver of group C showed a great improvement compared with group B which showed normal hepatic architecture consisting of normal hepatocytes, and normal sinusoids with normal other hepatic components except mild central vein dilation. The current study concludes that vitamin B12 helps to ameliorate the diazinon hepatotoxic effects as a model for a wide spread of organophosphorus compounds in agriculture and veterinary sectors by ameliorating its harmful oxidation on the liver since vitamin B12 is cheap and available as a commercial supplement therefore we recommend its uses
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