Integrative Metabolomic and Transcriptomic Analyses Reveal the Impact of Methionine Supplementation to Gibel Carp (<i>Carassius auratus gibelio</i>)
2025
Yujie Liu | Rendong Qian | Qiyou Xu | Jianhua Zhao
The experiment was conducted to evaluate the molecular mechanism of methionine supplementation on the growth of gibel carp. In the study, the following five groups were included: the control group (FM) was fed with a high-plant protein diet as basal diet, and four treatment groups were supplemented with 0.25% crystalline methionine (CM<sub>50</sub>), 0.50% crystalline methionine (CM<sub>100</sub>), 0.25% coated methionine (HM<sub>50</sub>) or 0.50% coated methionine (HM<sub>100</sub>), respectively. Each group consisted of four replicates of 25 fish each. The weight gain rate and specific growth rate of gibel carp in the HM<sub>100</sub> group were significantly higher than the FM group. The hepatopancreas transcriptomic (n = 4) and metabolomic (n = 6) analysis for the FM group and HM<sub>100</sub> group showed that the significantly differential metabolites mainly related to amino acid metabolism, protein digestion and absorption, and aminoacyl-tRNA biosynthesis. Additionally, some genes that were significantly different in these two groups were involved in energy metabolism and transmembrane transporter activity. Therefore, the improvement of fish growth by 0.50% coated methionine supplementation might be achieved through altering amino acid and energy-related metabolism in hepatopancreas, which provides new insights for understanding the impact of methionine on the growth of fish.
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