Preparation and characterization of a milk polar lipids enriched ingredient from fresh industrial liquid butterserum
2015
Gassi, Jean-Yves | Blot, Marielle | Beaucher, Eric | Robert, Benoit | Leconte, Nadine | Camier, Benedicte | Rousseau, Florence | Bourlieu-Lacanal, Claire | Jardin, Julien | Briard-Bion, Valérie | Lambert, Sophie | Gésan-Guiziou, Geneviève | Lopez, Christelle | Gaucheron, Frederic | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | French National Agency for Research (ANR) under the ALID program (Valobab; ANR-11-ALID-007) and by CNIEL.
interesting nutritional and functional properties. This is mainly due to technological difficulties for their concentration and purification from by-products of the dairy industry. The objective of this study was to develop a technological process able to provide a milk PLs enriched ingredient from industrial fresh liquid butterserum, with possibilities to valorize the by-products. The process comprised the following successive steps: skimming, heat treatment, acid precipitation of caseins, concentration and purification of whey butterserum by ultrafiltration and diafiltration. The proposed process yields to a recovery of 62% of PLs present in the initial butterserum. Thefinal ingredient contained 31.5 ± 1.5 and 46.9 ± 2.2 % of PLs on dry matter and fat contents basis, respectively. The proportions of the different PLs found in the final product were 7.2, 8.4, 20.9, 26.8 and 36.5 % for phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin, respectively. This fraction contained also proteins and triacylglycerols (about 26 and 34 % on a dry matter basis).The identified proteins were the 4caseins, whey proteins (α-lactalbumin, b-lactoglobulin, serum abumin, lactotransferrin) and 9 proteins from the milk fat globule membrane. This process will allow the preparation of milkPLs rich ingredient for food applications.
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