Effect of exogenous melatonin application on the expression of Catalase, Superoxide dismutase and Menthol Biosynthesis genes in Mentha pulegium L.
2025
Fatemeh, Ghasemi | Saideh, Khademi | Laleh, Arzi
Mentha pulegium L., an aromatic culinary herb, has been prevalent since antiquity for its variant pharmacological potencies. Melatonin, a versatile signalling compound, ameliorates the growth of various plants in counter to a variety of abiotic and biotic stresses via enhancing antioxidant machinery. The present survey, unprecedently, investigated the effect of exogenous melatonin application on the expression of catalase, superoxide dismutase, limonene synthase and menthol dehydrogenase. After the treatment of Mentha pulegium L with melatonin (0, 150 and 250 ?M) for 3 weeks, the expression levels of genes were evaluated by Real-time PCR. Our results illustrated that exogenous melatonin remarkably augmented mRNA expression of CAT, 5.64 and 29.22 fold changes respectively, for 150 and 250 ?M. The SOD expression was boosted by 4.11 and 19.66 fold corresponding to 150 and 250 ?M of melatonin. Also, exposure to melatonin upregulated the expression of genes that cooperated with menthol biosynthesis, Limonene synthase and Menthol dehydrogenase. 150 and 250 ?M of melatonin enhanced Limonene synthase expression by 3.10 and 17.89 folds and significant overexpression of menthol dehydrogenase was displayed for 150 and 250 ?M of melatonin, respectively by 13.80 and 23.32 times. Our findings propose melatonin can boost the oxidative stress resistance of Mentha pulegium L. by upgrading both the enzymatic and nonenzymatic antioxidant protective systems. Moreover, it elevated menthol production, which is in of demand in diverse industries, from pharmaceutical and cosmetics to tobacco and food processing.
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