Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer (Allium hirtifolium Boiss.) | Spremenljivost antioksidacijskega in antibakterijskega delovanja celokupnih fenolnih izvlečkov iz čebulic perzijske šalotke (Allium hirtifolium Boiss.)

Inglés. Allium hirtifolium Boiss. (mooseer) belonging to the family Alliaceae, is an endemic species of Iran which grows wild in the Zagros Mountains range, western and southwestern Iran. The bulb of A. hirtifolium has been used as a flavouring agent, especially dairy foods and pickles by the indigenous people, southwestern Iran. In this study, the bulbs of various populations of the plant were collected from the alpine regions in Chaharmahal va Bakhtiari province, Iran. The total phenolic content of the ethanol extract was determined by Folin– Ciocalteu method, the antioxidant activity was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the antibacterial activity of the extracts against four bacteria, including Bacillus cereus, Listeria monocytogenes, Proteus vulgaris, and Salmonella typhimurium was determined by serial dilution assay. Results indicated that the total phenolic content in the ethanol extracts of different populations of A. hirtifolium ranged between 34 to 44 mg gallic acid/g extract. In addition, the extracts of A. hirtifolium indicated moderate– to–good inhibitory activities (MICs = 0.062 to 0.250 mg/ml) against four bacteria, especially against B. cereus. The antioxidant activity of the bulbs of A. hirtifolium indicated the extract acted as an effective DPPH scavenger, but were not as effective as the BHT control. This finding suggests that the bulbs of A. hirtifolium may be considered as a natural source of antioxidants and antimicrobial agents.

Esloveno. Perzijska šalotka (Allium hirtifolium Boiss. (mooseer), Allium stipitatum Regel) spada v družino lukovk (Alliaceae), je endemična vrsta Irana, ki raste samoniklo v zahodnem in jugozahodnem delu države na območju gorovja Zagros. Prebivalci jugozahodnega Irana jo uporablja kot začimbo v mlečnih izdelkih in vlaganju zelenjave. V tej raziskavi so bile analizirane čebulice različnih populacij, nabrane v alpinskih predelih province Chaharmahal va Bakhtiari. Celokupna vsebnost fenolov je bila določena v etanolnem izvlečku po metodi Folin–Ciocalteu, antioksidativno delovanje je bilo ovrednoteno in izmerjeno z 1,1-difenil-2-pikrilhidrazilom (DPPH), antibakterijsko delovanje izvlečkov je bilo določeno proti štirim vrstam bakterij, Bacillus cereus, Listeria monocytogenes, Proteus vulgaris, in Salmonella typhimurium s serijskim razredčitvenim testom. Rezultati so pokazali, da so etanolni izvlečki celokupnih fenolov iz čebulic različnih populacij te vrste vsebovali od 34 do 44 mg galične kisline na g izvlečka. Izvlečki so pokazali zmerno do dobro inhibitorno aktivnost (MICs = 0.062 do 0.250 mg/ml) proti omenjenim štirim vrstam bakterij, še posebej proti vrsti B. cereus. Antioksidativno delovanje izvlečkov čebulic je pokazalo, da so izvlečki delovali kot učinkoviti lovilci DPPH, vendar so bili manj učinkoviti kot BHT v kontroli. Izsledki kažejo, da so lahko čebulice perzijske šalotke (A. hirtifolium) dober naravni vir antioksidantov in antimikrobnih snovi.