Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions | Aklimatizacija terestične orhideje Bletilla striata Rchb.f. (Orchidaceae) razmnožene v in vitro razmerah

Inglés. Bletilla striata is a terrestrial sympodial orchid. Substrates used for outdoor growing, differing in the mixture of added components and nutrients, were chosen for acclimatization of the asymbiotic propagated plants. A total of 651 Bletilla striata orchids were planted in 3 commercial substrates: ″Tonsubstrat″ (Ton), ″Baltisches substrat″ (Baltski) and ″Royal-Garden″ (Royal). Prior to acclimatization, the plants were 2.5 cm on average, with at least 2 leaves and 2 - 3 cm long root or roots. Fewest plants (3.1%) died in Ton substrate, 3.2% in Baltski substrate and 5.0% in Royal substrate. There were no statistically significant differences among substrates (p = 0.558) in the percentage of plants that died. Substrates used in combination with the chosen phenophase and established conditions were suitable for acclimatization of Bletilla striata orchids, whereby 95-97% of plants successfully adapted from heterotrophic to autotrophic conditions in a very short period of two months. The basic conditions for success are that plants are large and vital enough prior to acclimatization, that the substrate is appropriate and that appropriate conditions of relative humidity, temperature, light and ventilation without major fluctuations of these factors are ensured during acclimatization.

Esloveno. Bletilla striata je simpodialno razraščajoča se, v tleh rastoča orhideja. Za aklimatizacijo asimbiotsko razmnoženih rastlin smo izbrali substrate, ki se uporabljajo za gojenje rastlin na prostem ter se razlikujejo glede mešanice dodanih komponent in hranil. Skupno je bilo posajenih 651 orhidej v 3 komercialne substrate, ″Tonsubstrat″ (Ton), ″Baltisches substrat″ (Baltski) in ″Royal-Garden″ (Royal). Pred aklimatizacijo so imele rastline v povprečju 2,5 cm velik nadzemni del z vsaj dvema listoma in 2 do 3 cm dolgo korenino oz. korenine. Najmanj (3,1%) rastlin je propadlo v postopku aklimatizacije v Ton substratu, 3,2% v Baltskem substratu in največ 5,0% v Royal substratu. Med substrati ni bilo statistično značilnih razlik (p = 0,558) v odstotku propadlih rastlin med aklimatizacijo. Uporabljeni substrati v kombinaciji z izbrano fenofazo in vzpostavljene razmere so zelo primerni za aklimatizacijo te orhideje  saj se je 95 do 97% rastlin uspešno prilagodilo iz heterotrofnih na avtotrofne razmere in to v zelo kratkem obdobju dveh mesecev. Osnovni pogoj uspeha je, da so in vitro rastline pred aklimatizacijo dovolj velike in vitalne, primeren substrat ter ustrezne razmere, vlaga, temperatura, svetloba in kroženje zraka, brez večjih nihanj v obdobju aklimatizacije.